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His sound designs for theater have also been heard at the American Conservatory Theatre in San Francisco, The Public Theater in NY and the La Jolla Playhouse and the Old Globe Theatre in San Diego. LeBrecht started his career in the theater, working as the resident sound designer at the Berkeley Repertory Theatre for 10 years. Jim’s list of film credits (over 145) can be viewed at Other credits include The Waiting Room, The Devil and Daniel Johnston, Daughter From Danang, Have You Heard From Johannesburg, We Were Here, Bang: the Bert Berns Story, and Audrie and Daisy. BSA was proud to be the sound house for The Blood of Yingzhou District, which won the Academy Award for short documentary in 2006. Films that he has mixed have screened at film festivals including Sundance, Tribeca, South by Southwest, True/False, Telluride and Berlin. Known as a skillful sound designer and mixer, LeBrecht’s work can be heard in films that have played on HBO, PBS, and at the Rock and Roll Hall of Fame. Jim is the founder of Berkeley Sound Artists (BSA), an audio postproduction house, located in Berkeley, California. We need to tell our own stories.” He’s pushing his chair as hard as he can to make this happen. Jim believes that “the time has come for us to make our own films and television shows. Jim is currently a board member at the Disability Rights Education & Defense Fund, a leading organization working for the rights of the disabled through education, legislation and litigation. In recent years LeBrecht has recommitted to advocacy work, which had taken a back seat to his audio career. While in college at UC, San Diego, he helped found the Disabled Students Union. Jim’s work as an advocate for the rights of people with disabilities began in high school as a member of Disabled in Action, a pioneering disability rights group. James LeBrecht, Producer & Director, has over 35 years of experience as a film and theater sound designer and mixer, author, producer and disability rights activist. Twitter: /unrestfilm A middle-aged man smiles, He has brown curly hair, a grey goatee and wears a vintage looking cocktail hour shirt. Unrest is a vulnerable and eloquent personal documentary that is sure to hit closer to home than many could imagine. Jennifer Brea’s wonderfully honest portrayal asks us to rethink the stigma around an illness that affects millions of people. Utilizing Skype and social media, she unlocks a forgotten community with intimate portraits of four other families suffering similarly. Often confined by her illness to the private space of her bed, Jen is moved to connect with others around the globe. In this story of love and loss, newlyweds Jennifer and Omar search for answers as they face unexpected obstacles with great heart. (commonly known as Chronic Fatigue Syndrome), a mysterious illness some still believe is “all in your head.” Hoping to shed light on her strange symptoms, Jennifer grabs a camera and films the darkest moments unfolding before her eyes as she is derailed by M.E. Jennifer Brea is an active Harvard PhD student about to marry the love of her life when suddenly her body starts failing her. An activist for invisible disabilities and chronic illness, she co-founded a global advocacy network, #MEAction and is a TED Talker. She is also co-creator of Unrest VR, winner of the Sheffield Doc/Fest Alternate Realities Award. Her feature documentary, Unrest, premiered at the Sundance Film Festival in January, where it won a Special Jury Prize. In the aftermath, she rediscovered her first love, film. She has an AB from Princeton University and was a PhD student at Harvard until sudden illness left her bedridden. Jennifer Brea is an independent documentary filmmaker based in Los Angeles. She is looking slightly away from the camera and wearing a short-sleeved red blouse. About Headshot of Jen Brea, a young woman with curly brown hair with a wide smile. ‘Unrest’ Director Turns Camera On Herself To Document Chronic Fatigue Syndrome. A Place at the Table: Doc Filmmakers with Disabilities on Building Careers and Disproving Stereotypes. Documentary Magazine. Today’s episode features a conversation with two filmmakers: Jen Brea of ‘Unrest’ and Jim LeBrecht of ‘Crip Camp.’ Jen and Jim talk about their films, the power of disability narratives, the importance of documenting our disability communities and cultures, and how the filmmaking community needs to be more inclusive. There are plenty of documentaries about disabled people but very few are directed, written, or produced by disabled people. The game’s gore, action, and graphics are remarkable. One of my personal favorites on the site is WTF 76, it’s the best zombie shooter game I’ve ever played. 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Both animals do have a dorsal fin the dolphin fish’s dorsal fin runs along most of its body, while the dolphin's dorsal fin is in the center of its back. Is a dolphin a fish? No! The dolphin is a mammal, which is warm blooded, and has live births. How Is a Dolphin Fish Different From a Dolphin?ĭespite the name, dolphin fish and dolphins have virtually nothing in common, other than that they both swim. They’re usually caught by fishermen or predators. The fish can live up to five years, but it’s rare for them to exceed four, and the average lifespan is estimated to be 2-3 years. They usually swim north as waters warm, and populations in those northern areas peak around July. Dolphin fish don’t have strict migratory patterns. They are sometimes jokingly referred to as “the rabbits of the ocean” Dolphin fish reach maturity at 4-5 months old, and should definitely be fully mature by the time they’re a year old.įemale dolphin fish can lay 100,000-1,000,000 eggs at a time and can lay eggs 2-3 times per year. The dolphin fish is also found in large numbers off the coast of Hawaii.ĭolphin fish reproduce at a rapid rate. Dolphin fish are especially prominent in the Caribbean and the Gulf of Mexico, where a majority of fishing takes place. They can usually be found in depths up to 250 feet. While mahi mahi fish are most common in and prefer tropical waters, they can also be found in cooler waters as far north as Massachusetts.ĭolphin fish stay near the surface of the water, and close to the shore. They dwell in temperatures that are at least 90 degrees, usually on or near the equator. Mahi mahi fish grow to be 2.5-4.5 feet long, and catches have been recorded from 15 pounds to over 40 pounds.ĭolphin fish are found in the Indian, Pacific, and Atlantic Oceans in tropical and subtropical waters. The dolphin fish is also a vibrant, brightly colored fish that is golden on the sides and brightly colored blue and green on its sides and back. Males’ heads are rounded to the point where they protrude out. For the sake of this article, we’ll be focusing on the common dolphin fish.ĭolphin fish are a large species of fish that have a single dorsal fin that extends from the head to the tail. The two species are very similar, but the pompano dolphin fish is a bit smaller. There are actually two species of dolphin fish: the common dolphin fish, scientific name Coryphaena hippurus, and the pompano dolphin fish, Coryphaena equiselis. The dolphin fish is a brightly colored, vibrant fish that is an important part of ocean ecosystems. The dolphin fish, like dolphins, are predators, and mainly roam the seas in the Gulf of Mexico, the Caribbean, and Hawaii. While the dolphin fish is not actually a dolphin, it is a large, strong, fish that has a dorsal fin, which may inspire some comparisons to dolphins. A dolphin fish, also known as mahi-mahi, is a constant in tropical waters and, dare we say it, on seafood menus. MIT's license for SnapGene requires that users log in from one of the following list of IP address ranges: Cisco An圜onnect client for Windows, Mac OS X and Linux.How to connect to MIT's Virtual Private Network If you are accessing SnapGene from an off campus location, you will need to connect to the MIT Virtual Private Network before launching SnapGene. MIT's license for SnapGene requires that users log in from specified MIT networks, including the MIT campus network, CSAIL, the legacy AI lab address space, Lincoln, Haystack, LNS, and the PSFC or from an MIT VPN connection. SnapGene is licensed for educational, academic and research use by the MIT community. When performing a diagnostic restriction digest of your minipreps, always include the starting vector as a reference standard.How can I login to SnapGene from an off campus location? Access SnapGene from specified MIT networks.If you obtain many more transformants for the ligation with the inserted fragment than for the control ligation, your cloning almost certainly worked, and you can usually get away with making just 3-4 minipreps.If the DNA has any blunt ends, ligate at room temperature for ~4 hr and use high-concentration T4 ligase. If the DNA has only sticky ends, ligate at room temperature for ~1 hr.coli efficiently, and re‑circularization of the vector cannot occur without ligation to a phosphorylated fragment. This control ligation should yield very few transformants because only circular DNA molecules transform E. With a phosphatase-treated vector, perform a control ligation in which the inserted fragment is omitted.If you plan to clone a blunt-ended PCR fragment (generated with a polymerase such as Pfu) into a phosphatase-treated vector, ensure that your PCR primers have 5′-phosphates. If the inserted fragment was generated by PCR, purify it with a gel or spin-column before doing any restriction digests.When visualizing DNA bands with a transilluminator, do not use short wavelength UV light of 312 nm or below because the DNA will be severely damaged. In addition to removing unwanted or incompletely digested DNA, this procedure removes enzymes and small molecules. Purify the inserted fragment with a gel.You can use relatively short digestion times, and for a double digest, you can use a reaction buffer that is suboptimal for one or both enzymes. For preparation of an inserted fragment, incomplete digestion is not a serious concern because partial recovery of the fragment is acceptable.It is a good idea to spin briefly after vortexing to ensure that no droplets are left on the side of the tube. Vortex the mixtures thoroughly and repeatedly, and do not allow any droplets of liquid to escape enzyme treatment. With the vector, ensure that the digestion and phosphatase reactions go to completion. Gel purification of the vector is usually unnecessary because phosphatase treatment will inactivate any extra DNA fragments that were generated. Remove the phosphatase with a spin-column.After digesting the vector (and blunting, if appropriate), treat with phosphatase: 1 hr at 37☌ for 5′ overhangs, or 1 hr at 50☌ if the DNA has any blunt ends or 3′ overhangs.If the vector needs to be cut with two enzymes that have different optimal reaction buffers, perform the digests sequentially, with a spin-column purification in between. Use an excess of restriction enzyme (~20 U for 2 μg DNA), and digest for ~4 hr. Ensure that digestion of the vector goes to completion.The tricks described below will minimize these effects. This problem has two causes: incomplete digestion of the vector, and re‑ligation of the cut vector with itself. The most common problem with restriction cloning is that the starting vector is recovered after the procedure. Cleaner starting material will yield a better outcome. When in doubt, purify a DNA fragment with a gel.For example, it works better to clone a blunt-ended fragment into a blunt vector site, such as a SmaI site, than into a site that has been blunted with Klenow or T4 DNA polymerase. To maximize efficiency, minimize the number of steps in a procedure.(There is no need to use a spin-column before purifying a DNA fragment with a gel.) Elute the DNA in 40 – 45 μl of 10 mM Tris (pH 8.5), and then perform the next reaction. After each enzymatic reaction, purify the DNA with a spin-column.Start with about 2 μg of DNA when preparing a vector or excising a fragment to be inserted. This approach saves time in the long run. First and foremost, be careful at each step of a procedure.A few simple tricks will help to ensure that your cloning goes smoothly. Restriction Cloning Tipsįor many applications, conventional restriction cloning is still the best method. Using a specific example, you will learn to simulate inserting a single fragment into a plasmid vector using restriction and insertion cloning in SnapGene. Just Download, Run Setup And James Bond 007: NightFire ( PC, 2002) Case and game, tested, fast Downloads for advanced or expert users Nightfire Dedicated Server Project 007 is a new James Bond game (Image credit: IO Interactive) All told, the initial Project 007 Named 99). James bond pc game 2012 free download You don’t knowwhy –. Get stock market quotes, personal finance advice, company news and more. 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Sleep is vital for virtually all animals. Gringras' team concluded: 'Ideally future software design could be better optimized when night-time use is anticipated, and hardware should allow an automatic "bedtime mode" that shifts blue and green light emissions to yellow and red as well as reduce backlight/light intensity.' Both approaches were effective at reducing blue light. The researchers then tested two strategies for limiting light emissions: wearing orange-tinted (blue-blocking) safety glasses, and using an app called 'Kids Sleep Dr' that adjusts a device's color palette. This confirmed that modern screens give-off blue wavelengths. In his recent study, Gringras and colleagues compared light emitted from various devices – an iPhone 5s, iPad Air and Kindle Paperwhite e-reader – when displaying text. NEXT: Why a 'bedtime mode' improves your health. "People fall asleep later and they wake-up with more inertia – more grogginess, probably reflecting the fact that you've not slept for quite as long as you need." Work pressures force adults to get an early start or finish late, for example, while children catch-up on social media before school so they aren't behind on gossip.Īs a result, Gringras argues that there's no slack in the system. The artificial light problem is made worse by our busy modern lives, which push sleep patterns to the limit. Using a laptop in bed (Image CC 0: Pixabay) And while the invention of the light bulb has had many benefits for human society, it's also created some adverse effects on our health. Stimulation from artificial light at inappropriate times stops the body clock from becoming synchronized with the outside world. "You'd need to drink a lot of coffee to postpone sleep onset and melatonin production as much as bright light exposure would." 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Human physiology and behavior during a 24-hour cycle (Image: Wikimedia)Įyes sense light via photoreceptor cells in the retina, using 'cones' for colour vision and 'rods' for black-and-white. The rise and fall of melatonin levels over a 24-hour period influences your physiology and behavior, including blood pressure and general alertness. The sleep hormone is destroyed by sunlight, so melatonin is sometimes known as a ' Dracula hormone'. This master body clock then prompts the nearby pineal gland to release melatonin, a hormone that circulates in the blood and tells your cells it's time to sleep. Light is detected by the eye, which relays that stimulus to the 'suprachiasmatic nucleus' within the hypothalamus, a cluster of neurons in the middle of the brain. Clocks can be adjusted by an external cue, what biologists call a zeitgeber (German for 'time-giver'), and sunlight is nature's most powerful cue. 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